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Targeted Protein Surface Sensors as a Tool for Analyzing Small Populations of Proteins in Biological Mixtures
Author(s) -
Motiei Leila,
Pode Zohar,
Koganitsky Anna,
Margulies David
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201402501
Subject(s) - biomarker , computational biology , gene isoform , nanotechnology , protein array analysis , surface protein , protein detection , computer science , chemistry , biology , materials science , biochemistry , gene , gene expression , dna microarray , virology
Optical cross‐reactive sensor arrays (the so‐called chemical “noses/tongues”) have recently been demonstrated as a powerful tool for high‐throughput protein detecting and analysis. Nevertheless, applying this technology to biomarker detection is complicated by the difficulty of non‐selective sensors to operate in biological mixtures. Herein we demonstrate a step toward circumventing this limitation by using self‐assembled fluorescent receptors consisting of two distinct recognition motifs: specific and non‐specific. When combined in an array, binding cooperatively between the specific and non‐specific protein binders enables the system to discriminate among closely related isoform biomarkers even in the presence of serum proteins or within human urine.