Premium
Catching Bubbles: Targeting Ultrasound Microbubbles Using Bioorthogonal Inverse‐Electron‐Demand Diels–Alder Reactions
Author(s) -
Zlitni Aimen,
Janzen Nancy,
Foster F. Stuart,
Valliant John F.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201402473
Subject(s) - bioorthogonal chemistry , microbubbles , tetrazine , on demand , computer science , ultrasound , chemistry , nanotechnology , combinatorial chemistry , materials science , click chemistry , medicine , radiology , multimedia , organic chemistry
Catching bubbles : Tetrazine‐functionalized microbubbles were prepared for use as ultrasound contrast agents, and shown to selectively localize on cells labeled with a TCO‐derivatized antibody against VEGFR2. This capture approach based on labeling and bioorthogonal chemistry was validated in a flow‐chamber assay and in vivo through ultrasound imaging of VEGFR2‐positive and VEGFR2‐negative murine tumor models. TCO= trans ‐cyclooctene.