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Cytoprotective Silica Coating of Individual Mammalian Cells through Bioinspired Silicification
Author(s) -
Lee Juno,
Choi Jinsu,
Park Ji Hun,
Kim MiHee,
Hong Daewha,
Cho Hyeoncheol,
Yang Sung Ho,
Choi Insung S.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201402280
Subject(s) - hela , jurkat cells , allylamine , coating , cell , regenerative medicine , chemistry , cell culture , nanotechnology , materials science , biophysics , biochemistry , biology , polyelectrolyte , polymer , immunology , organic chemistry , t cell , immune system , genetics
The cytoprotective coating of physicochemically labile mammalian cells with a durable material has potential applications in cell‐based sensors, cell therapy, and regenerative medicine, as well as providing a platform for fundamental single‐cell studies in cell biology. In this work, HeLa cells in suspension were individually coated with silica in a cytocompatible fashion through bioinspired silicification. The silica coating greatly enhanced the resistance of the HeLa cells to enzymatic attack by trypsin and the toxic compound poly(allylamine hydrochloride), while suppressing cell division in a controlled fashion. This bioinspired cytocompatible strategy for single‐cell coating was also applied to NIH 3T3 fibroblasts and Jurkat cells.