Bidirectional Regulation of mRNA Translation in Mammalian Cells by Using PUF Domains
Cao Jicong,
Arha Manish,
Sudrik Chaitanya,
Schaffer David V.,
Kane Ravi S.
Publication year2014
Publication title
angewandte chemie international edition
Resource typeJournals
Abstract The regulation of gene expression is crucial in diverse areas of biological science, engineering, and medicine. A genetically encoded system based on the RNA binding domain of the Pumilio and FBF (PUF) proteins was developed for the bidirectional regulation (i.e., either upregulation or downregulation) of the translation of a target mRNA. PUF domains serve as designable scaffolds for the recognition of specific RNA elements and the specificity can be easily altered to target any 8‐nucleotide RNA sequence. The expression of a reporter could be varied by over 17‐fold when using PUF‐based activators and repressors. The specificity of the method was established by using wild‐type and mutant PUF domains. Furthermore, this method could be used to activate the translation of target mRNA downstream of PUF binding sites in a light‐dependent manner. Such specific bidirectional control of mRNA translation could be particularly useful in the fields of synthetic biology, developmental biology, and metabolic engineering.
Subject(s)biology , computational biology , downregulation and upregulation , gene , gene expression , genetics , messenger rna , microbiology and biotechnology , mutant , protein biosynthesis , repressor , rna , rna binding protein , synthetic biology , translation (biology) , translational regulation
SCImago Journal Rank5.831

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