Premium
Engineering the Stereochemistry of Cephalosporin for Specific Detection of Pathogenic Carbapenemase‐Expressing Bacteria
Author(s) -
Shi Haibin,
Cheng Yunfeng,
Lee Kyung Hyun,
Luo Robert F.,
Banaei Niaz,
Rao Jianghong
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201402012
Subject(s) - cephalosporin , enterobacteriaceae , microbiology and biotechnology , bacteria , recombinant dna , chemistry , pathogenic bacteria , carbapenem , beta lactamase inhibitors , biology , enzyme , escherichia coli , biochemistry , gene , antibiotics , genetics
Reported herein is the design of fluorogenic probes specific for carbapenem‐resistant Enterobacteriaceae (CRE) and they were designed based on stereochemically modified cephalosporin having a 6,7‐ trans configuration. Through experiments using recombinant β‐lactamase enzymes and live bacterial species, these probes demonstrate the potential for use in the specific detection of carbapenemases, including metallo‐β‐lactamases in active bacterial pathogens.