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Riboswitches Based on Kissing Complexes for the Detection of Small Ligands
Author(s) -
Durand Guillaume,
Lisi Samuele,
Ravelet Corinne,
Dausse Eric,
Peyrin Eric,
Toulmé JeanJacques
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201400402
Subject(s) - aptamer , ligand (biochemistry) , surface plasmon resonance , folding (dsp implementation) , riboswitch , small molecule , biophysics , loop (graph theory) , chemistry , biosensor , rna , crystallography , computational biology , nanotechnology , biology , materials science , biochemistry , receptor , genetics , gene , mathematics , engineering , combinatorics , nanoparticle , electrical engineering , non coding rna
Biosensors derived from aptamers were designed for which folding into a hairpin shape is triggered by binding of the cognate ligand. These aptamers (termed aptaswitches) thus switch between folded and unfolded states in the presence and absence of the ligand, respectively. The apical loop of the folded aptaswitch is recognized by a second hairpin called the aptakiss through loop–loop or kissing interactions, whereas the aptakiss does not bind the unfolded aptaswitch. Therefore, the formation of a kissing complex signals the presence of the ligand. Aptaswitches were designed that enable the detection of GTP and adenosine in a specific and quantitative manner by surface plasmon resonance when using a grafted aptakiss or in solution by anisotropy measurement with a fluorescently labeled aptakiss. This approach is generic and can potentially be extended to the detection of any molecule for which hairpin aptamers have been identified, as long as the apical loop is not involved in ligand binding.