Premium
Live‐Cell Stimulated Raman Scattering Imaging of Alkyne‐Tagged Biomolecules
Author(s) -
Hong Senlian,
Chen Tao,
Zhu Yuntao,
Li Ang,
Huang Yanyi,
Chen Xing
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201400328
Subject(s) - bioorthogonal chemistry , biomolecule , raman scattering , raman spectroscopy , microscopy , fluorescence , fluorescence lifetime imaging microscopy , chemistry , live cell imaging , nanotechnology , biophysics , materials science , click chemistry , cell , biochemistry , combinatorial chemistry , biology , optics , physics
Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within the Raman‐silent region of a cell. Coupling this spectroscopic signature with Raman microscopy yields a new imaging modality beyond fluorescence and label‐free microscopies. The bioorthogonal Raman imaging of various biomolecules tagged with an alkyne by a state‐of‐the‐art Raman imaging technique, stimulated Raman scattering (SRS) microscopy, is reported. This imaging method affords non‐invasiveness, high sensitivity, and molecular specificity and therefore should find broad applications in live‐cell imaging.