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Rapid Generation of Cell Gradients by Utilizing Solely Nanotopographic Interactions on a Bio‐Inert Glass Surface
Author(s) -
Yang Gao,
Cao Yanhua,
Fan Junbing,
Liu Hongliang,
Zhang Feilong,
Zhang Pengchao,
Huang Chao,
Jiang Lei,
Wang Shutao
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201309974
Subject(s) - filopodia , cell , substrate (aquarium) , materials science , inert , nanotechnology , biophysics , chemistry , biology , ecology , biochemistry , organic chemistry
The control of cell gradients is critical for understanding many biological systems and realizing the unique functionality of biomimetic implants. Herein, we report a nanotopographic gradient strategy that can rapidly generate cell gradients on a nanodendritic silica substrate without any chemical modification. We can achieve controllable cell gradients within only half an hour of cell incubation solely induced by the topographic effect of the gradient nanodendrites. We also demonstrate that cell gradients can be modulated by the combination of nanotopographic and chemical gradients. The results reveal that the enhanced topographic interactions between the nanodentritic structure and nanoscaled filopodia of the cells mainly contribute to the generation of cell gradients.