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Three‐Dimensional Protein Networks Assembled by Two‐Photon Activation
Author(s) -
Gatterdam Volker,
Ramadass Radhan,
Stoess Tatjana,
Fichte Manuela A. H.,
Wachtveitl Josef,
Heckel Alexander,
Tampé Robert
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201309930
Subject(s) - glutathione , two photon excitation microscopy , multiplexing , chemistry , biophysics , nanotechnology , excitation , materials science , physics , computer science , biochemistry , biology , enzyme , quantum mechanics , telecommunications
Abstract Spatial and temporal control over chemical and biological processes plays a key role in life and material sciences. Here we synthesized a two‐photon‐activatable glutathione (GSH) to trigger the interaction with glutathione S‐transferase (GST) by light at superior spatiotemporal resolution. The compound shows fast and well‐confined photoconversion into the bioactive GSH, which is free to interact with GST‐tagged proteins. The GSH/GST interaction can be phototriggered, changing its affinity over several orders of magnitude into the nanomolar range. Multiplexed three‐dimensional (3D) protein networks are simultaneously generated in situ through two‐photon fs‐pulsed laser‐scanning excitation. The two‐photon activation facilitates the three‐dimensional assembly of protein structures in real time at hitherto unseen resolution in time and space, thus opening up new applications far beyond the presented examples.