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Back Cover: Acetylcholinesterase‐Catalyzed Hydrolysis Allows Ultrasensitive Detection of Pathogens with the Naked Eye (Angew. Chem. Int. Ed. 52/2013)
Author(s) -
Liu Dingbin,
Wang Zhantong,
Jin Albert,
Huang Xinglu,
Sun Xiaolian,
Wang Fu,
Yan Qiang,
Ge Shengxiang,
Xia Ningshao,
Niu Gang,
Liu Gang,
Hight Walker A. R.,
Chen Xiaoyuan
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201309874
Subject(s) - analyte , naked eye , acetylcholinesterase , hydrolysis , cover (algebra) , chemistry , combinatorial chemistry , chromatography , detection limit , enzyme , biochemistry , mechanical engineering , engineering
A new colorimetric assay for pathogen detection is described by X. Chen et al. in their Communication on page 14065 ff. An improved heterogeneous sandwich‐type ELISA enhances detection sensitivity by a two‐step signal amplification process involving gold particle aggregation induced by acetylcholinesterase‐catalyzed hydrolysis, which leads to a color change, followed by enrichment of the analyte using magnetic beads. This assay is as sensitive as RT‐PCR, but more convenient and less expensive.