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Protein Engineering from “Scratch” Is Maturing
Author(s) -
Höhne Matthias,
Bornscheuer Uwe T.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201309591
Subject(s) - protein engineering , scratch , protein design , substrate (aquarium) , scaffold , scaffold protein , enzyme , active site , natural (archaeology) , computer science , chemistry , protein structure , biochemistry , biology , ecology , programming language , signal transduction , paleontology
Precisely tuning the active site by protein engineering has led to the development of a highly efficient Kemp eliminase (see structure with substrate in the binding pocket). The starting protein scaffold with only low activity originated from computational design, as no natural enzyme with this activity was known. This is a breakthrough in protein design, as novel catalytic activities are now in reach that match those of natural enzymes.