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Identification of Living Legionella pneumophila Using Species‐Specific Metabolic Lipopolysaccharide Labeling
Author(s) -
Mas Pons Jordi,
Dumont Audrey,
Sautejeau Grégory,
Fugier Emilie,
Baron Aurélie,
Dukan Sam,
Vauzeilles Boris
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201309072
Subject(s) - legionella pneumophila , legionella , microbiology and biotechnology , lipopolysaccharide , bacteria , identification (biology) , biology , outbreak , virology , immunology , ecology , genetics
Legionella pneumophila is a pathogenic bacterium involved in regular outbreaks characterized by a relatively high fatality rate and an important societal impact. Frequent monitoring of the presence of this bacterium in environmental water samples is necessary to prevent these epidemic events, but the traditional culture‐based detection and identification method requires up to 10 days. Reported herein is a method allowing identification of Legionella pneumophila by metabolic lipopolysaccharide labeling which targets, for the first time, a precursor to monosaccharides that are specifically present within the O‐antigen of the bacterium. This new approach allows easy detection of living Legionella pneumophila , while other Legionella species are not labeled.