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Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells
Author(s) -
Xiao Han,
Chatterjee Abhishek,
Choi Seihyun,
Bajjuri Krishna M.,
Sinha Subhash C.,
Schultz Peter G.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201308137
Subject(s) - computer science , computational biology , fluorophore , amino acid , tumor cells , chemistry , biochemistry , biology , fluorescence , physics , cancer research , quantum mechanics
An enhanced suppression system enables the expression of proteins in mammalian cells incorporating one unnatural amino acid (UAA) into multiple sites, as well as two different UAAs into distinct sites in a protein of interest. The utility of this technology was demonstrated by generating a full‐length antibody, site‐specifically conjugated to a drug and a fluorophore, and characterizing its activity in vitro (see picture).