Inhibition of Heme Uptake in Pseudomonas aeruginosa by its Hemophore (HasA p ) Bound to Synthetic Metal Complexes

The heme acquisition system A protein secreted by Pseudomonas aeruginosa (HasA p ) can capture several synthetic metal complexes other than heme. The crystal structures of HasA p harboring synthetic metal complexes revealed only small perturbation of the overall HasA p structure. An inhibitory effect upon heme acquisition by HasA p bearing synthetic metal complexes was examined by monitoring the growth of Pseudomonas aeruginosa PAO1. HasA p bound to iron–phthalocyanine inhibits heme acquisition in the presence of heme‐bound HasA p as an iron source.