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Detection and Characterization of 1,2‐Dibromoethane‐Derived DNA Crosslinks Formed with O 6 ‐Alkylguanine‐DNA Alkyltransferase
Author(s) -
Chowdhury Goutam,
Cho SungHee,
Pegg Anthony E.,
Guengerich F. Peter
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201307580
Subject(s) - dna , characterization (materials science) , mutagenesis , computational biology , chemistry , computer science , combinatorial chemistry , biochemistry , biology , nanotechnology , gene , mutation , materials science
A combination of chemical modifications and LC‐tandem MS was used for the structure elucidation of various ethylene crosslinks of DNA with O 6 ‐alkylguanine‐DNA alkyltransferase (AGT, see picture). The elucidation of the chemical structures of such DNA–protein crosslinks is necessary to understand mechanisms of mutagenesis.