Premium
Imaging the Glycosylation State of Cell Surface Glycoproteins by Two‐Photon Fluorescence Lifetime Imaging Microscopy
Author(s) -
Belardi Brian,
de la Zerda Adam,
Spiciarich David R.,
Maund Sophia L.,
Peehl Donna M.,
Bertozzi Carolyn R.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201307512
Subject(s) - glycoprotein , glycosylation , glycan , fluorescence microscope , fluorescence , microscopy , computer science , visualization , computational biology , focus (optics) , multiphoton fluorescence microscope , chemistry , information retrieval , biochemistry , biology , artificial intelligence , physics , optics
Glycoproteins in focus : Metabolic labeling of glycans with azido sugars (see picture) in combination with two‐photon fluorescence lifetime imaging microscopy enables the visualization of specific glycoforms of endogenous proteins. This method can be utilized to detect glycosylated proteins in both cell culture and intact human tissue slices.