Premium
Minimum Set of Cytochromes P450 for Reconstituting the Biosynthesis of Camalexin, a Major Arabidopsis Antibiotic
Author(s) -
Klein Andrew P.,
AnaratCappillino Gülbenk,
Sattely Elizabeth S.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201307454
Subject(s) - arabidopsis , biosynthesis , antibiotics , biology , chemistry , genetics , gene , mutant
Bringing it all together : The missing key step in the biosynthesis of camalexin was uncovered by in vitro biochemical characterization. The coupling of Trp‐ and Cys‐derived fragments through CS bond formation (see scheme, right) is promoted by an unusual cytochrome P450 CYP71A13. The in vitro reconstitution of the camalexin biosynthesis (left) from Trp and Cys was achieved using just three cytochromes P450. IAN=indole‐3‐acetonitrile.