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Noninvasive Measurement of the Mechanical Force Generated by Motor Protein EF‐G during Ribosome Translocation
Author(s) -
Yao Li,
Li Yue,
Tsai TeWie,
Xu Shoujun,
Wang Yuhong
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201307419
Subject(s) - dissociation (chemistry) , ribosome , messenger rna , chromosomal translocation , computer science , motor protein , computational biology , chemistry , biophysics , biology , microbiology and biotechnology , gene , rna , biochemistry , microtubule
The power stroke of a motor protein : The motor protein EF‐G generates a power stroke of 89 pN during ribosome translocation (see picture). This mechanical force is obtained by measuring the force‐induced dissociation of a series of DNA–mRNA duplexes. The dissociation is indicated by a decrease in magnetic signal.