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Molecular Characterization of DNA Double Strand Breaks with Tip‐Enhanced Raman Scattering
Author(s) -
Lipiec Ewelina,
Sekine Ryo,
Bielecki Jakub,
Kwiatek Wojciech M.,
Wood Bayden R.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201307271
Subject(s) - dna , raman scattering , dna damage , raman spectroscopy , deoxyribose , fluorescence , biophysics , fluorescence microscope , chemistry , cleavage (geology) , microscopy , atomic force microscopy , nanoscopic scale , materials science , nanotechnology , optics , biology , physics , biochemistry , fracture (geology) , composite material
DNA double strand breaks (DSBs) are deadly lesions that can lead to genetic defects and cell apoptosis. Techniques that directly detect DNA DSBs include scanning electron microscopy, atomic force microscopy (AFM), and fluorescence based approaches. While these techniques can be used to identify DSBs they provide no information on the molecular events occurring at the break. Tip‐enhanced Raman scattering (TERS) can provide molecular information from DNA at the nanoscale and in combination with AFM provides a new way to visualize and characterize the molecular structure of DSBs. DSBs result from cleavage at the 3’‐ and 5’‐bonds of deoxyribose upon exposure to UVC radiation based on the observation of POH and methyl/methylene deformation modes enhanced in the TERS spectra. It is hypothesized that strand fragments are hydrogen‐terminated at the lesion, indicating the action of free radicals during photon exposure.