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And the Winner is…︁Azadithiolate: An Amine Proton Relay in the [FeFe] Hydrogenases
Author(s) -
Schilter David,
Rauchfuss Thomas B.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201307132
Subject(s) - hydrogenase , active site , chemistry , relay , amine gas treating , enzyme , combinatorial chemistry , biochemistry , physics , organic chemistry , power (physics) , quantum mechanics
A victory in the pocket : An international team of chemists and biophysicists have resolved the long‐standing question of the structure of the active site of the [FeFe] hydrogenases by assembling the active enzyme with a version of the active site synthesized in vitro (see scheme; HydF is a scaffold protein, HydA1 is a natural hydrogenase). The protein incorporating the diiron complex 2 showed similar activity to that of the natural enzyme.