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Mechanically Modulating the Photophysical Properties of Fluorescent Protein Biocomposites for Ratio‐ and Intensiometric Sensors
Author(s) -
Brantley Johnathan N.,
Bailey Constance B.,
Can Joe R.,
Clark Katie A.,
Vanden Bout David A.,
Brodbelt Jennifer S.,
KeatingeClay Adrian T.,
Bielawski Christopher W.
Publication year - 2014
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201306988
Subject(s) - hypsochromic shift , fluorescence , green fluorescent protein , quenching (fluorescence) , biophysics , aggregation induced emission , materials science , rational design , composite number , polymer , nanotechnology , photochemistry , chemistry , biochemistry , composite material , optics , biology , physics , gene
Mechanically sensitive biocomposites comprised of fluorescent proteins report stress through distinct pathways. Whereas a composite containing an enhanced yellow fluorescent protein (eYFP) exhibited hypsochromic shifts in its fluorescence emission maxima following compression, a composite containing a modified green fluorescent protein (GFPuv) exhibited fluorescence quenching under the action of mechanical force. These ratio‐ and intensiometric sensors demonstrate that insights garnered from disparate fields (that is, polymer mechanochemistry and biophysics) can be harnessed to guide the rational design of new classes of biomechanophore‐containing materials.