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Aptamer‐Guided Caging for Selective Masking of Protein Domains
Author(s) -
Rohrbach Falk,
Schäfer Florian,
Fichte Manuela A. H.,
Pfeiffer Franziska,
Müller Jens,
Pötzsch Bernd,
Heckel Alexander,
Mayer Günter
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201306686
Subject(s) - aptamer , computer science , linker , masking (illustration) , information retrieval , biology , microbiology and biotechnology , programming language , art , visual arts
Lightly unmasked : Large protein subdomains can be masked and photolytically released using aptamers with a suitable photocleavable linker. No structural knowledge of the protein site is needed. Thrombin Exosite I activity can be efficiently regulated with this approach (see scheme), allowing light‐controlled activation of blood clotting.