Premium
Development of an Activity‐Based Probe and In Silico Design Reveal Highly Selective Inhibitors for Diacylglycerol Lipase‐α in Brain
Author(s) -
Baggelaar Marc P.,
Janssen Freek J.,
van Esbroeck Annelot C. M.,
den Dulk Hans,
Allarà Marco,
Hoogendoorn Sascha,
McGuire Ross,
Florea Bogdan I.,
Meeuwenoord Nico,
van den Elst Hans,
van der Marel Gijsbert A.,
Brouwer Jaap,
Di Marzo Vincenzo,
Overkleeft Herman S.,
van der Stelt Mario
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201306295
Subject(s) - diacylglycerol lipase , in silico , diacylglycerol kinase , lipase , computational biology , biochemistry , chemistry , biology , enzyme , gene , protein kinase c
A model method : A strategy that combines a knowledge‐based in silico design approach and the development of novel activity‐based probes (ABPs) for the detection of endogenous diacylglycerol lipase‐α (DAGL‐α) is presented. This approach resulted in the rapid identification of new DAGL‐α inhibitors with high selectivity in the brain proteome. ABPP=activity‐based protein profiling.