Premium
Biosynthesis of the Antimetabolite 6‐Thioguanine in Erwinia amylovora Plays a Key Role in Fire Blight Pathogenesis
Author(s) -
Coyne Sébastien,
Chizzali Cornelia,
Khalil Mohammed N. A.,
Litomska Agnieszka,
Richter Klaus,
Beerhues Ludger,
Hertweck Christian
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201305595
Subject(s) - fire blight , erwinia , biology , antimetabolite , pathogenesis , microbiology and biotechnology , biosynthesis , heterologous , bacteria , biochemistry , genetics , gene , immunology , chemotherapy
Sulfur for fire : The molecular basis for the biosynthesis of the antimetabolite 6‐thioguanine (6TG) was unveiled in Erwinia amylovora , the causative agent of fire blight. Bioinformatics, heterologous pathway reconstitution in E. coli , and mutational analyses indicate that the protein YcfA mediates guanine thionation in analogy to 2‐thiouridylase. Assays in planta and in cell cultures reveal for the first time a crucial role of 6TG in fire blight pathogenesis.