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Gadolinium(III) Spin Labels for High‐Sensitivity Distance Measurements in Transmembrane Helices
Author(s) -
Matalon Erez,
Huber Thomas,
Hagelueken Gregor,
Graham Bim,
Frydman Veronica,
Feintuch Akiva,
Otting Gottfried,
Goldfarb Daniella
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201305574
Subject(s) - gadolinium , transmembrane protein , transmembrane domain , electron paramagnetic resonance , nuclear magnetic resonance , sensitivity (control systems) , chemistry , site directed spin labeling , flexibility (engineering) , membrane , biophysics , crystallography , physics , biochemistry , biology , mathematics , receptor , statistics , organic chemistry , electronic engineering , engineering
High‐frequency double electron‐electron resonance (DEER) distance measurements using different Gd 3+ tags (Gd‐DOTA and Gd‐C1) were carried out on transmembrane helical peptides (ca. 0.15 nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins.