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Multiplex Detection of DNA Mutations by the Fluorescence Fingerprint Spectrum Technique
Author(s) -
Song Jinzhao,
Zhang Jiangyan,
Lv Fengting,
Cheng Yongqiang,
Wang Bing,
Feng Liheng,
Liu Libing,
Wang Shu
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201305461
Subject(s) - multiplex , förster resonance energy transfer , dna , fingerprint (computing) , fluorescence , multiplex polymerase chain reaction , mutation , microbiology and biotechnology , genetics , biology , gene , polymerase chain reaction , physics , computer science , optics , artificial intelligence
A fingerprint spectrum technique that utilizes cationic conjugated‐polymer‐based fluorescence resonance energy transfer (FRET) is used for multiplex detection of DNA mutations. This method detects as low as 5 % mutation of the total DNA. Ten PIK3CA mutations originating from 30 clinical breast cancer samples could be detected.

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