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Cysteine Promoted C‐Terminal Hydrazinolysis of Native Peptides and Proteins
Author(s) -
Adams Anna L.,
Cowper Ben,
Morgan Rachel E.,
Premdjee Bhavesh,
Caddick Stephen,
Macmillan Derek
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201304997
Subject(s) - cysteine , terminal (telecommunication) , chemistry , biochemistry , computer science , enzyme , telecommunications
Tagging the terminus : N→S acyl transfer in native peptides and proteins can be reliably intercepted with hydrazine. The method allows selective labeling and ligation, without recourse to the use of protein‐splicing elements. NCL=native chemical ligation.