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Fluorogenic ATP Analogues for Online Monitoring of ATP Consumption: Observing Ubiquitin Activation in Real Time
Author(s) -
Hacker Stephan M.,
Pagliarini Dana,
Tischer Thomas,
Hardt Norman,
Schneider Daniel,
Mex Martin,
Mayer Thomas U.,
Scheffner Martin,
Marx Andreas
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201304723
Subject(s) - ubiquitin , ubiquitin ligase , biochemistry , enzyme , deubiquitinating enzyme , chemistry , cysteine , adenosine triphosphate , ubiquitin conjugating enzyme , microbiology and biotechnology , biology , gene
Many enzymes use ATP in signal‐transducing processes or as an energy source. New fluorogenic ATP analogues signal ATP consumption by ubiquitin‐like protein‐activating enzymes in real time. Thus the inhibition and stimulation of these ATP‐processing enzymes can be studied without auxiliary enzymes and reagents. β‐Lapachone was identified as an inhibitor of the ubiquitin‐activating enzyme UBA1 (see scheme; A=acceptor, D=donor).