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Photodissociation and Dissociative Photoionization Mass Spectrometry of Proteins and Noncovalent Protein–Ligand Complexes
Author(s) -
Ca Francis,
Milosavljević Aleksandar R.,
van der Rest Guillaume,
Réfrégiers Matthieu,
Nahon Laurent,
SarniManchado Pascale,
Cheynier Véronique,
Giuliani Alexandre
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201304046
Subject(s) - photoionization , chemistry , photodissociation , mass spectrometry , fragmentation (computing) , tandem mass spectrometry , tandem mass tag , non covalent interactions , protein ligand , electron capture dissociation , proteomics , photochemistry , molecule , chromatography , quantitative proteomics , ionization , ion , biochemistry , biology , ecology , hydrogen bond , organic chemistry , gene
Weight of evidence : Using synchrotron radiation, photo‐fragmentation of an intrinsically disordered protein is probed and compared with classical tandem mass‐spectrometry activation techniques. It provides excellent sequence coverage allowing the identification of the protein noncovalent binding sites.