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DNA Modification under Mild Conditions by Suzuki–Miyaura Cross‐Coupling for the Generation of Functional Probes
Author(s) -
Lercher Lukas,
McGouran Joanna F.,
Kessler Benedikt M.,
Schofield Christopher J.,
Davis Benjamin G.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201304038
Subject(s) - dna , chemistry , reactivity (psychology) , combinatorial chemistry , covalent bond , ligand (biochemistry) , suzuki reaction , epigenetics , biochemistry , catalysis , organic chemistry , palladium , receptor , medicine , alternative medicine , pathology , gene
Quick and clean : A method for Pd‐catalyzed Suzuki–Miyaura cross‐coupling to iododeoxyuridine (IdU) in DNA is described. Key to the reactivity is the choice of the ligand and the buffer. A covalent [Pd]–DNA intermediate was isolated and characterized. Photocrosslinking probes were generated to trap proteins that bind to epigenetic DNA modifications.