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Enhanced Accuracy of Single‐Molecule Diffusion Measurements with a Photocleavable Energy‐Transfer Dyad
Author(s) -
Dill Maren,
Baier Moritz C.,
Mecking Stefan,
Wöll Dominik
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201303658
Subject(s) - dyad , molecule , diffusion , förster resonance energy transfer , energy transfer , chemistry , fluorescence , range (aeronautics) , tracking (education) , energy (signal processing) , chemical physics , analytical chemistry (journal) , biological system , materials science , optics , physics , thermodynamics , organic chemistry , quantum mechanics , acoustics , composite material , chromatography , biology , psychology , pedagogy
A photocleavable energy‐transfer dyad was synthesized, characterized, and applied to single‐molecule fluorescence microscopy. After photocleavage, a combination of independent two‐color single‐molecule tracking and analysis of single‐molecule energy‐transfer efficiencies allows the determination of the temporal evolution of the relative distances between both fragments from the nm to the μm scale. This gives access to a broad range of diffusion coefficients.