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Expanding the Genetic Code for Photoclick Chemistry in E. coli , Mammalian Cells, and A. thaliana
Author(s) -
Li Fahui,
Zhang Hua,
Sun Yun,
Pan Yanchao,
Zhou Juanzuo,
Wang Jiangyun
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201303477
Subject(s) - arabidopsis thaliana , genetic code , transfer rna , mutant , organism , in vitro , computational biology , biology , model organism , code (set theory) , genetics , computer science , rna , microbiology and biotechnology , chemistry , dna , gene , programming language , set (abstract data type)
Breaking the (genetic) code : N ‐ε‐acryl‐ lysine was genetically incorporated into proteins in bacterial cells, mammalian cells, and Arabidopsis thaliana by co‐expressing a pyrrolysyl‐tRNA mutant synthetase and its cognate tRNA in the model organism. A photoclick reaction driven by irradiation by 365 nm UV light or 405 nm laser light and allowed for site‐specific protein labeling within minutes both in vitro and in vivo.