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Stabilization of Peptides for Intracellular Applications by Phosphoramidate‐Linked Polyethylene Glycol Chains
Author(s) -
Nischan Nicole,
Chakrabarti Alokta,
Serwa Remigiusz A.,
BoveeGeurts Petra H. M.,
Brock Roland,
Hackenberger Christian P. R.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201303467
Subject(s) - phosphoramidate , pegylation , intracellular , polyethylene glycol , jurkat cells , computer science , chemistry , peg ratio , computational biology , lysis , biochemistry , biology , t cell , immune system , genetics , finance , economics
PEG intracellularly! Although long known to enhance residence half‐life of peptides in serum and lysates, the effect of PEGylation on biological probes in cells has received only limited attention. Here it is shown that phosphoramidate‐linked PEGylated proapoptotic peptides display a dramatically increased stability in Jurkat cell lysate and a homogenous intracellular distribution as well as high apoptotic activity after introduction into cells.