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N‐Terminal Labeling of Peptides by Trypsin‐Catalyzed Ligation for Quantitative Proteomics
Author(s) -
Pan Yanbo,
Ye Mingliang,
Zhao Liang,
Cheng Kai,
Dong Mingming,
Song Chunxia,
Qin Hongqiang,
Wang Fangjun,
Zou Hanfa
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201303429
Subject(s) - trypsin , proteomics , proteome , quantitative proteomics , dna ligase , biochemistry , protease , chemistry , computational biology , enzyme , computer science , biology , gene
Two times an enzyme : Trypsin was first used as a protease to catalyze the digestion of proteins and then as a ligase to catalyze the linking of isotopically labeled amino acids to the N termini of tryptic peptides for quantitative proteomics. Reliable and accurate quantification of proteins was demonstrated for model proteins as well as proteome samples.