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Back Cover: A Programmable Sensor to Probe the Internalization of Proteins and Nanoparticles in Live Cells (Angew. Chem. Int. Ed. 22/2013)
Author(s) -
Liu Haiyin,
Johnston Angus P. R.
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201303271
Subject(s) - internalization , nanosensor , fluorescence , nanotechnology , nanoparticle , biophysics , dna , cover (algebra) , chemistry , materials science , cell , biochemistry , biology , physics , optics , engineering , mechanical engineering
The internalization of proteins and nanoparticles in living cells can be quantified by using a new DNA nanosensor, as described by A. P. R. Johnston and H. Liu in their Communication on page 5744 ff. The material of interest is labeled with a fluorescent DNA probe, and the fluorescence of any material that remains on the surface of the cell is quenched upon the addition of a quencher probe. The fluorescence of internalized material and other fluorophores is unaffected. This technique enables multi‐color assays and studies in primary cells without compromising sensitivity or quantification.