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A Unique Family of Rigid Analogues of the GFP Chromophore with Tunable Two‐Photon Action Cross‐Sections for Biological Imaging
Author(s) -
Yuan Lin,
Lin Weiying,
Chen Hua,
Zhu Sasa,
He Longwei
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201303179
Subject(s) - chromophore , green fluorescent protein , fluorescence , two photon excitation microscopy , action (physics) , in vitro , chemistry , photon , combinatorial chemistry , nanotechnology , computer science , photochemistry , materials science , physics , biochemistry , optics , gene , quantum mechanics
Conformationally restricted analogues of the GFP chromophore have been synthesized. The spectroscopic properties of GCTPOC, a unique family with tunable two‐photon action cross‐sections, were investigated. GCTPOC could be used as a robust two‐photon platform for development of a two‐photon fluorescent thiol sensor, which could stain endogenous thiols both in vitro and in vivo.