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Chemoenzymatic Synthesis of the Immunoglobulin Domain of Tim‐3 Carrying a Complex‐Type N‐Glycan by Using a One‐pot Ligation
Author(s) -
Asahina Yuya,
Kamitori Shigehiro,
Takao Toshifumi,
Nishi Nozomu,
Hojo Hironobu
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201303073
Subject(s) - glycan , thioester , domain (mathematical analysis) , ligation , chemistry , computer science , peptide , computational biology , combinatorial chemistry , biochemistry , stereochemistry , microbiology and biotechnology , biology , enzyme , mathematics , glycoprotein , mathematical analysis
Ligations : The title immunoglobin (Ig) domain was chemoenzymatically synthesized. The domain was divided into three segments and N‐ and S‐protected peptide thioesters were synthesized by solid‐phase peptide synthesis. The one‐pot sequential ligation was achieved by using Ag + ‐free and Ag + ‐assisted activation of the thioester. After folding and enzymatic transfer of the synthetic glycan, the desired Ig domain carrying a nonasaccharide was successfully obtained.