Premium
A Chemo‐Enzymatic Approach for Site‐Specific Modification of the RNA Cap
Author(s) -
Schulz Daniela,
Holstein Josephin Marie,
Rentmeister Andrea
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201302874
Subject(s) - bioorthogonal chemistry , click chemistry , chemistry , methionine , enzyme , combinatorial chemistry , biochemistry , thiol , stereochemistry , rna , amino acid , gene
Capped and gowned : A two‐step approach can be used to site‐specifically modify the 5′‐cap of eukaryotic mRNAs. First, a trimethylguanosinesynthase variant recognizes the m 7 G cap structure and introduces bioorthogonal groups using S ‐adenosyl‐ L ‐methionine‐based cosubstrates. Then, the enzymatically introduced reporter groups are further modified by thiol–ene or CuAAC click chemistry (see scheme).