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Light‐Responsive Helical Polypeptides Capable of Reducing Toxicity and Unpacking DNA: Toward Nonviral Gene Delivery
Author(s) -
Yin Lichen,
Tang Haoyu,
Kim Kyung Hoon,
Zheng Nan,
Song Ziyuan,
Gabrielson Nathan P.,
Lu Hua,
Cheng Jianjun
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201302820
Subject(s) - cationic polymerization , transfection , dna , gene delivery , chemistry , unpacking , internalization , biophysics , helix (gastropod) , microbiology and biotechnology , biochemistry , gene , biology , cell , ecology , linguistics , philosophy , organic chemistry , snail
Gene delivery vehicles : Helical, cationic polypeptides with light‐responsive domains showed potent membrane activity and promoted efficient cellular internalization of DNA (see picture). After transfection, a light trigger induces protecting‐group removal to expose anionic carboxylate groups. The reduced cationic charge and helix distortion of the polypeptides result in enhanced DNA unpacking and reduced material toxicity because of the eliminated membrane activity.