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Rigid DNA Beams for High‐Resolution Single‐Molecule Mechanics
Author(s) -
Pfitzner Emanuel,
Wachauf Christian,
Kilchherr Fabian,
Pelz Benjamin,
Shih William M.,
Rief Matthias,
Dietz Hendrik
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201302727
Subject(s) - dna , optical tweezers , molecule , dna origami , bridging (networking) , resolution (logic) , high resolution , materials science , nanotechnology , biophysics , optics , chemistry , physics , biology , computer science , biochemistry , computer network , remote sensing , artificial intelligence , geology , organic chemistry
Bridging the gap : Rigid DNA linkers (blue, see picture) between microspheres (green) for high‐resolution single‐molecule mechanical experiments were constructed using DNA origami. The resulting DNA helical bundles greatly reduce the noise generated in studies of conformation changes using optical tweezers and were applied to study small DNA secondary structures.