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Improved Super‐Resolution Microscopy with Oxazine Fluorophores in Heavy Water
Author(s) -
Lee Steven F.,
Vérolet Quentin,
Fürstenberg Alexandre
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201302341
Subject(s) - fluorescence , resolution (logic) , brightness , microscopy , quantum yield , heavy water , fluorescence microscope , materials science , chemistry , optics , physics , computer science , deuterium , quantum mechanics , artificial intelligence
Brighter dyes in heavy water : A simple and cost‐effective method increases the brightness of a whole class of commonly used red‐emitting fluorophores, including ATTO655, ATTO680, and ATTO700. Replacing water (H 2 O) by heavy water (D 2 O) in the imaging buffer doubles the fluorescence quantum yield of these dyes and significantly improves the localization precision in super‐resolution imaging.