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Thermostable Branched DNA Nanostructures as Modular Primers for Polymerase Chain Reaction
Author(s) -
Hartman Mark R.,
Yang Dayong,
Tran Thua N. N.,
Lee Kwang,
Kahn Jason S.,
Kiatwuthi Pichamon,
Yancey Kenneth G.,
Trotsenko Oleksandr,
Minko Sergiy,
Luo Dan
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201302175
Subject(s) - polymerase chain reaction , dna , chemistry , polymerase , combinatorial chemistry , inverse polymerase chain reaction , hot start pcr , nanostructure , dna polymerase , modular design , nanotechnology , microbiology and biotechnology , nested polymerase chain reaction , materials science , biology , biochemistry , gene , computer science , operating system
Chemical cross‐linking was used to prepare DNA nanostructures with enhanced thermal stability. These thermostable DNA nanostructures were then utilized as modular primers in the polymerase chain reaction (PCR; see picture), thus enabling the production of multifunctionalized and branched PCR products for multiplexed detection and hydrogel formation.