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Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA
Author(s) -
Abe Naoko,
Hiroshima Michio,
Maruyama Hideto,
Nakashima Yuko,
Nakano Yukiko,
Matsuda Akira,
Sako Yasushi,
Ito Yoshihiro,
Abe Hiroshi
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201302044
Subject(s) - rna , translation (biology) , ribosome , computer science , circular rna , service (business) , open reading frame , computational biology , biology , genetics , gene , messenger rna , peptide sequence , economy , economics
Getting the runaround : Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell‐free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis.