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Two‐Color Probe to Monitor a Wide Range of pH Values in Cells
Author(s) -
Lee Min Hee,
Han Ji Hye,
Lee Jae Hong,
Park Nayoung,
Kumar Rajesh,
Kang Chulhun,
Kim Jong Seung
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201301894
Subject(s) - rhodamine , confocal , fluorescein , wavelength , confocal microscopy , chemistry , optics , analytical chemistry (journal) , microscopy , materials science , biophysics , optoelectronics , fluorescence , physics , chromatography , biology
High‐tech pH paper : A “chameleon” pH probe composed of rhodamine (red, see scheme) and fluorescein (green) units emits at wavelengths of 580 nm and 512 nm, where the intensities show a contrary response to pH changes. Confocal microscopy of HeLa cells with this probe reveals red and green spots; the ratio of these signals can be calibrated to give the pH value of the respective organelle.