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Top‐Down Chemoenzymatic Approach to a High‐Mannose‐Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming
Author(s) -
Koizumi Akihiko,
Matsuo Ichiro,
Takatani Maki,
Seko Akira,
Hachisu Masakazu,
Takeda Yoichi,
Ito Yukishige
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201301613
Subject(s) - trimming , mannose , glycan , enzyme , chemistry , combinatorial chemistry , biochemistry , computer science , glycoprotein , programming language
From the stacks : A novel method for construction of a high‐mannose‐type glycan library by systematic enzymatic trimming of a single synthetic Man 9 ‐based precursor was developed. Efficient chemical synthesis of the tetradecasaccharide common precursor and orthogonal enzymatic trimming to obtain all M 8‐9 and G 1 M 8‐9 derivatives was demonstrated. G=glucose, M=mannose.