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Repair of the (6–4) Photoproduct by DNA Photolyase Requires Two Photons
Author(s) -
Yamamoto Junpei,
Martin Ryan,
Iwai Shigenori,
Plaza Pascal,
Brettel Klaus
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201301567
Subject(s) - photolyase , pyrimidine dimer , photon , dimer , oxetane , dna , chemistry , dna repair , physics , photochemistry , optics , biochemistry , nuclear magnetic resonance , polymer chemistry
It takes two (photons) to tango : Single‐turnover flash experiments showed that the flavoenzyme (6–4) photolyase uses a successive two‐photon mechanism to repair the UV‐induced T(6–4)T lesion in DNA (see picture). The intermediate (X) formed by the first photoreaction is likely to be the oxetane‐bridged dimer T(ox)T. The enzyme could stabilize the normally short‐lived T(ox)T, allowing repair to be completed by the second photoreaction.