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Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence
Author(s) -
Reddington Samuel C.,
Rizkallah Pierre J.,
Watson Peter D.,
Pearson Rachel,
Tippmann Eric M.,
Jones D. Dafydd
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201301490
Subject(s) - green fluorescent protein , azide , fluorescence , genetic code , protein function , computer science , function (biology) , chemistry , combinatorial chemistry , computational biology , photochemistry , gene , biology , biophysics , biochemistry , genetics , physics , organic chemistry , quantum mechanics
Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.