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The Structure of FemX Wv in Complex with a Peptidyl‐RNA Conjugate: Mechanism of Aminoacyl Transfer from Ala‐tRNA Ala to Peptidoglycan Precursors
Author(s) -
Fonvielle Matthieu,
Li de La SierraGallay Inés,
ElSagheer Afaf H.,
Lecerf Maxime,
Patin Delphine,
Mellal Dénia,
Mayer Claudine,
Blanot Didier,
Gale Nittaya,
Brown Tom,
van Tilbeurgh Herman,
EthèveQuelquejeu Mélanie,
Arthur Michel
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201301411
Subject(s) - transfer rna , rna , mechanism (biology) , conjugate , computational biology , peptidoglycan , ribosomal rna , chemistry , stereochemistry , combinatorial chemistry , biology , biochemistry , gene , physics , mathematical analysis , mathematics , quantum mechanics
To gain insight into the catalytic mechanism of non‐ribosomal amino acid transferases, peptidyl‐RNA conjugates were synthesized for co‐crystallization with FemX Wv of Weissella viridescens , which transfers L ‐Ala from Ala‐tRNA Ala to the peptidoglycan precursor UDP‐MurNAc‐pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemX Wv binds its substrates for substrate‐assisted catalysis and stabilization of the tetrahedral intermediate.