Premium
Rapidly Reversible Manipulation of Molecular Activity with Dual Chemical Dimerizers
Author(s) -
Lin YuChun,
Nihongaki Yuta,
Liu TzuYu,
Razavi Shiva,
Sato Moritoshi,
Inoue Takanari
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201301219
Subject(s) - fkbp , relocation , dual (grammatical number) , event (particle physics) , computational biology , world wide web , computer science , chemistry , microbiology and biotechnology , biology , biochemistry , physics , linguistics , quantum mechanics , programming language , philosophy
Tell it where to go : Rapamycin induced the relocation of an FRB‐fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs–FKBP–C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs–FKBP–C2(LACT)/rapamycin/FRB–POI complex to the Tom20–GID1‐labeled mitochondria with the termination of POI‐dependent signaling.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom