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Rapidly Reversible Manipulation of Molecular Activity with Dual Chemical Dimerizers
Author(s) -
Lin YuChun,
Nihongaki Yuta,
Liu TzuYu,
Razavi Shiva,
Sato Moritoshi,
Inoue Takanari
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201301219
Subject(s) - fkbp , relocation , dual (grammatical number) , event (particle physics) , computational biology , world wide web , computer science , chemistry , microbiology and biotechnology , biology , biochemistry , physics , linguistics , quantum mechanics , programming language , philosophy
Tell it where to go : Rapamycin induced the relocation of an FRB‐fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs–FKBP–C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs–FKBP–C2(LACT)/rapamycin/FRB–POI complex to the Tom20–GID1‐labeled mitochondria with the termination of POI‐dependent signaling.