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Development of a MALDI‐TOF MS Strategy for the High‐Throughput Analysis of Biomarkers: On‐Target Aptamer Immobilization and Laser‐Accelerated Proteolysis
Author(s) -
Zhang Xueyang,
Zhu Shaochun,
Xiong Ya,
Deng Chunhui,
Zhang Xiangmin
Publication year - 2013
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201300566
Subject(s) - aptamer , proteolysis , lysozyme , covalent bond , chemistry , chromatography , throughput , nanotechnology , biophysics , materials science , biochemistry , microbiology and biotechnology , enzyme , biology , telecommunications , organic chemistry , computer science , wireless
An aptamer‐based strategy was developed for the high‐throughput analysis of protein biomarkers, such as lysozyme, by on‐target MALDI‐TOF MS. The aptamers were immobilized on the target plate through formation of covalent bonds with a stable and porous gold layer. An infrared laser was subsequently applied for fast proteolysis (see picture). High sensitivities were observed both in standard solutions and human urine.